NANO-BIO
NEO-LIVE
Innovative in vivo imaging probe
NEO-LIVE is the multimodal probe for in vivo live imaging of both
magnetic resonance (MR) and fluorescence imaging. It is a core-shell
structure (magnetic NP-silica), and a Near Infra-Red (NIR) fluorescent
dye is incorporated in a silica shell.
In particular, this probe is specially designed for improving the photostability
and controlling the fluorescent signal intensity. It can greatly be
powerful in vivo study and detected by in vivo imaging system.
Absorption and emission spectra of NEO-LIVETM Blue (NEO-LIVETM Magnoxide
675), Green (NEO-LIVETM Magnoxide 730), Red (NEO-LIVETM Magnoxide 797)
DEMONSTRATIONS OF IN VIVO IMAGING WITH NEO-LIVE
High sensitivity of NEO-LIVE (Detection limit test). Small number of A549 cells
labeled with NEO-LIVE (Magnoxide 797) was injected (A) subcutaneously for testing the in
vivo imaging detection limits. We were able to observe labeled cells with up to a minimal
numbers of 2.5×103. (B) Tried again under same condition of small numbers of injection.
In vivo images of mouse brain injected with U87MG cancer cells labeled by NEO-LIVE
(Magnoxide797). 5×105 U87MG cancer cells (labeling with NEO-LIVE (Magnoxide797)) were
injected into the brain for imaging the brain cancer growth.
In vivo image of mouse liver by NEO-LIVE (Magnoxide797). 7.5 × 104 hMSC cells
labeled with NEO-LIVE were injected into tail vein of BALB/C nude mouse, the image was taken
using MaestroTM In Vivo Imaging System.
In vivo images of a mouse articular capsule by NEO-LIVE (Magnoxide797) and signal
degradation graph. 1 × 106 chondrocyte labeled by NEO-LIVE (Magnoxide797) was injected
into the articular capsule, and then we detected. Increasing the time, fluorescence signal from
labeled cells was slowly decreased while that of dye itself was rapidly decreased.
In vivo images of a rat brain injected with NEO-LIVE (Magnoxide675). Once in vivo
live imaging was done, the injection site was sacrificed for clarifying the performance by NEOLIVE
(Magnoxide675).
